Primer Design and Search Tool

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Primer search, ePCR

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Statistics

Visitors:

1231730

Primer tm:

101830

Primer score:

22887/14740

Search:

18421/13757

Fast PCR:

3453835/1358896

Primer design:

111034/125753

MSP design:

37037

Version:

2.75

Last update:

28/09/24

Search Methylated Specific Primers

Sequence
Bisulfite	Use sense or antisense chain.
Set search region for	Forward primer: - Reverse primer: -
Max length of PCR
Min tm diff
CpG sites	let only in one or both primers.

Parameters

Primer melting temperature

Primer conc	mikromol	Glycerol conc	%
Potassium conc	milimol	Ethylen glycol conc	%
Magnesium conc	milimol	Formamid conc	%

Primer scoring values

Description	Weight	Min	Opt	Max		Description	Weight	Max
Primer length						Self annealing
GC content						Self end-annealing
GC content (bis)						Pair annealing
Melting temp						Pair end-annealing

Primer design

Results list size

Max Tm diff

Minimum of CpGs

Help

Search the best primer pairs for PCR a sequence

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