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Visitors:1231723
Primer tm:101830
Primer score:22887/14740
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Primer design:111034/125753
MSP design:37037
Version:2.75
Last update:28/09/24

Calculate melting temperature of a primer

Primer sequence

Parameters

Primer melting temperature
Primer conc  mikromol Glycerol conc  %
Potassium conc  milimol Ethylen glycol conc  %
Magnesium conc  milimol Formamid conc  %

Help

Calculating melting temperature of a primer

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