BiSearch: Primer Design and Search Tool

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Primer tm

Primer score

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Primer search, ePCR

Primer design

MSP design

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Visitors:1231714
Primer tm:101830
Primer score:22887/14740
Search:18421/13757
Fast PCR:3453831/1358896
Primer design:111034/125753
MSP design:37037
Version:2.75
Last update:28/09/24

Calculate score of a primer pair

Forward primer
Reverse primer
Bisulfite

Parameters

Primer melting temperature
Primer conc  mikromol Glycerol conc  %
Potassium conc  milimol Ethylen glycol conc  %
Magnesium conc  milimol Formamid conc  %
Primer scoring values
Description Weight Min Opt Max    Description Weight Max
Primer length Self annealing
GC content Self end-annealing
GC content (bis) Pair annealing
Melting temp Pair end-annealing

Help

Calculating melting temperature of a primer

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